HVEM Antibody [HMHV-1B18], Rabbit IgG

• Catalog Number: orb1671795
• Category: Antibodies
• Description: HVEM Antibody [HMHV-1B18], Rabbit IgG
• Clonality: Recombinant
• Clone Number: HMHV-1B18
• Tested applications: Blocking, FA, FC, IP, WB
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising Armenian hamsters with mouse HVEM:Fc fusion protein.
• Concentration: batch dependent
• Conjugation: Unconjugated
• UniProt ID: Q71F55
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD270, TNF Receptor-like 2, ATAR, Herpes virus Ent
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-HVEM antibody HVEM-1B18 (Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of HVEM-1B18 (orb1671795 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-HVEM antibody HMHV-1B18. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of HMHV-1B18 (orb1671795) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671795- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-HVEM antibody HMHV-1B18. Mouse spleen(A)- mouse thymus (B) and mouse lung(C) tissue lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of HMHV-1B18 (orb1671795) at 0.01 ug/ml- before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.