Human VEGF110 Protein

• Catalog Number: orb1496221
• Category: Proteins
• Description: Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 110,121,145,165,183,189 and 206 amino acids in length are expressed in humans.
• Reactivity: Human
• Tag: N-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 14.59 kDa
• Target: VEGF110
• Source: Human VEGF110 Protein, His Tag (orb1496221) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 136 (Accession # NP_001165097.1).
• Expression System: HEK293
• Biological Activity: Human VEGFR1/R2 captured on Protein A Chip can bind Human VEGF110 Protein, His Tag with an affinity constant of 13.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
• Expression Region: Ala 27 - Arg 136
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001165097.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 14.59 kDa. The protein migrates as 15 kDa and 18-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Human VEGFR1/R2 captured on Protein A Chip can bind Human VEGF110 Protein, His Tag (Cat. No. orb1496221) with an affinity constant of 13.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).