Human VEGF R3 / FLT4 Protein

• Catalog Number: orb18510
• Category: Proteins
• Description: Vascular endothelial growth factor receptor 3 (VEGF R3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). VEGF R3 mediates lymphangiogenesis in response to VEGF-C and VEGF-D. VEGF R3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGF R3 may be important for lymph angiogenesis.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 111.2 kDa
• Target: VEGF R3
• Source: Human VEGF R3, Fc Tag (orb18510) is expressed from human 293 cells (HEK293). It contains AA Tyr 25 - Ile 776 (Accession # NP_002011.2).
• Expression System: HEK293
• Biological Activity: Human VEGF R3, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Tyr 25 - Ile 776
• Endotoxins: 1.0 EU per μg
• NCBI: NP_002011.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH7.5
• Alternative names: Flt-4,FLT4,LMPH1A,PCLFLT41,VEGFR3,VEGFR-3,FLT-4,FL
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 111.2 kDa. As a result of glycosylation, the protein migrates as 140-160 kDa under reducing (R) condition, and 280-320 kDa under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human VEGF R3 protein

Human VEGF R3, Fc Tag (Cat. No. orb18510) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag (Cat. No. orb257928) with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).