Human VEGF-C / Flt4-L Protein

• Catalog Number: orb257927
• Category: Proteins
• Description: Vascular endothelial growth factor C is also known as VEGFC, Flt4-L and VRP, it contains the C-terminal propeptide which has an unusual structure with tandemly repeated cysteine-rich motifs. Upon biosynthesis, VEGFC is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGFC is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. The structure and function of this protein is similar to those of vascular endothelial growth factor D (VEGF-D). VEGFC may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Overexpression of VEGF-C causes lymphatics to enlarge possibly facilitates metastasis.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 14.9 kDa
• Target: VEGF-C
• Source: Human VEGF-C, His Tag (orb257927) is expressed from human 293 cells (HEK293). It contains AA Thr 103 - Arg 227 (Accession # AAH35212.1).
• Expression System: HEK293
• Biological Activity: Human VEGF-C, His Tag captured on CM5 Chip via anti-His antibody can bind Human VEGF R3, Fc Tag with an affinity constant of 41.1 pM as determined in SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Thr 103 - Arg 227
• Endotoxins: 1.0 EU per μg
• NCBI: AAH35212.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: VEGFC,Flt4-L,VRP
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.9 kDa. The protein migrates as 19-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

de Guzman, Roche C. et al. PEG-Immobilized Keratin for Protein Drug Sequestration and pH-Mediated Delivery J Drug Deliv, 2016, 7843951 (2016)

PubMed: 26904294

SDS-PAGE analysis of Human VEGF-C protein

Human VEGF-C, His Tag (Cat. No. orb257927) captured on CM5 Chip via anti-His antibody can bind Human VEGF R3, Fc Tag (Cat. No. orb18510) with an affinity constant of 41.1 pM as determined in SPR assay (Biacore T200) (Routinely tested).