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Human Uroplakin 2 (UPK2) ELISA Kit

Catalog Number: orb779081

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb779081
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Uroplakin 2(UPK2).
Tested applicationsELISA
ReactivityHuman
Range0.16-10 ng/mL
Assay Time3.5h
Concentration10 ng/mL
UniProt IDO00526
Sensitivity0.052 ng/mL
Alternative namesUPII ELISA Kit, Uroplakin II ELISA Kit
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NoteFor research use only
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human UPK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human UPK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human UPK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human UPK2 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typestissue homogenates, cell lysates and other biological fluids
Expiration DatePlease enquire.
Human Uroplakin 2 (UPK2) ELISA Kit