Human UCH-L1 Protein

Catalog Number: orb257912

Catalog Number	orb257912
Category	Proteins
Description	Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ,a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain. UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also,UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.
Reactivity	Human
Tag	N-6×His
Form/Appearance	Powder
Purity	95%
Conjugation	Unconjugated
MW	25.7 kDa
Target	UCH-L1
Protein Sequence	NP_004172
Source	Human UCH-L1, His Tag (orb257912) is expressed from E. coli cells. It contains AA Gln 2 - Ala 223 (Accession # NP_004172).
Expression System	E. coli
Expression Region	Gln 2 - Ala 223
Endotoxins	1.0 EU per μg
NCBI	NP_004172
Storage	-20℃
Buffer/Preservatives	50 mM Tris, 150 mM Nacl, pH8.0
Alternative names	UCHL1,PGP9.5 Read more...
Note	For research use only
Application notes	This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 25.7 kDa. The protein migrates as 27-28 kDa under reducing (R) condition (SDS-PAGE).
Expiration Date	6 months from date of receipt.

SDS-PAGE analysis of Human PGP9.5 protein

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 50.7 kDa after removal of the signal peptide.The apparent molecular mass of hFc-UCHL1 is approximately 35-55 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 100 μg, 10 μg, 50 μg

Human PGP9.5 protein [orb392670]
Applications:
ELISA, MS, SDS-PAGE, WB
Purity:
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Source:
E. coli
Sizes Available: 500 μg, 50 μg, 10 μg
Human PGP9.5 ELISA Kit [orb406174]
Reactivity:
Human
Dynamic Range:
6.25 ng/mL-400 ng/mL
Sensitivity:
1.56 ng/mL
Sizes Available: 10 x 96 t, 5 x 96 t, 96 t, 48 t, 24T
UCHL1 siRNA (Human) [orb1863237]
Applications:
RNA-I
Sizes Available: 30 nmol, 15 nmol

Applications:	ELISA, MS, SDS-PAGE, WB
Purity:	Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Source:	E. coli