Human Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

SKU: orb775055

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha(TNFa).

Research Area

Cancer Biology; Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFa in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.4 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

TNF Alpha, DIF ELISA Kit, TNF-A ELISA Kit, TNFSF2 ELISA Kit, Cachectin ELISA Kit, Tumor Necrosis Factor Ligand Superfamily Member 2 ELISA Kit

Quick Database Links

UniProt

P01375

Neşe Ayşit et al. N-Methyl-D-Aspartate Receptor (NMDAR) Antibodies Induce Diverse Calcium Activity Patterns on Cultured Astrocytes and Astrocyte–Neuron Cocultures Acta Neurologica, (2025)

PubMed:

Applications

ELISA

Reactivity

Human

Bożena Kowalczyk et al. N-Methylation of Legionaminic Acid in Legionella pneumophila Serogroup 1 LPS Affects Cell Surface Properties, Intracellular Growth, and Cytokine Response Pathogens ., (2026)

PubMed: 42347227

Applications

ELISA

Reactivity

Human