Human Tumor Necrosis Factor Alpha (TNFa) ELISA Kit

• Catalog Number: orb775055
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha(TNFa).
• Reactivity: Human
• Concentration: 1000 pg/mL
• UniProt ID: P01375
• Tested applications: ELISA
• Application notes: standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNFa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNFa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNFa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNFa in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.4 pg/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: TNF Alpha, DIF ELISA Kit, TNF-A ELISA Kit, TNFSF2
• Research Area: Cancer Biology, Immunology & Inflammation, Infecti
• Note: For research use only
• Expiration Date: Please enquire.

References

Neşe Ayşit et al. N-Methyl-D-Aspartate Receptor (NMDAR) Antibodies Induce Diverse Calcium Activity Patterns on Cultured Astrocytes and Astrocyte–Neuron Cocultures Acta Neurologica, (2025)

PubMed:

Applications

ELISA

Reactivity

Human