Human Troponin I Type 1, Slow Skeletal (TNNI1) ELISA Kit

• Catalog Number: orb777276
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I Type 1, Slow Skeletal(TNNI1).
• Reactivity: Human
• Concentration: 3000 pg/mL
• UniProt ID: P19237
• Tested applications: ELISA
• Application notes: standard: 3000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TNNI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TNNI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TNNI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TNNI1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 46.88-3000 pg/mL
• Sensitivity: 17.58 pg/mL
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Alternative names: TNN1 ELISA Kit, Troponin I, slow-twitch isoform EL
• Research Area: Cardiovascular Research
• Note: For research use only