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Catalog Number | orb80105 |
---|---|
Category | Proteins |
Description | TFF-2 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 106 amino acids (24-129) and having total molecular mass of 12 kDa. TFF2 Human Recombinant includes 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds and was purified by proprietary chromatographic techniques. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | TFF2 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized TFF2 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKSVEDCHY. |
Biological Origin | E.coli |
Biological Activity | Determined by its ability to chemoattract human MCF-7 cells using concentration 1.0-10.0 ng/ml. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The TFF2 protein was lyophilized from 0.4 um filtered solution at concentration of 1mg/mL containing 1x PBS pH-7.4. |
Alternative names | TFF-2 protein, Spasmolytic polypeptide protein, Sp Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. | |
Human cells |
Human | |
31.25 ng/mL-2000 ng/mL | |
7.81 ng/mL |