Human 4-1BBL

SKU: orb2994593

Description

Recombinant Human 4-1BB Ligand is produced by our E.coli expression system and the target gene encoding Arg71-Glu254 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: E.coli
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human 4-1BBL-His (Cat#orb2994593) at 10μg/ml (100 μl/well) can bind Human 4-1BB-Fc (Cat#orb2994312).The ED50 of Human 4-1BB-Fc (Cat#orb2994312) is 16.8 ng/ml.. BLI: Loaded Human 4-1BB-Fc-His(Cat#orb2994490) on Protein A Biosensor, can bind Human 4-1BBL-His(Cat#orb2994593) with an affinity constant of 1.55 pM as determined in BLI assay.
• Tag: C-6His
• Molecular Weight: Predicted: 20.6 KDa. Observed: 16-20 KDa, reducing conditions
• Expression Region: Arg71-Glu254
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Tumor necrosis factor ligand superfamily member 9; 4-1BB ligand; 4-1BBL; TNFSF9

Quick Database Links

UniProt

P41273