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Catalog Number | orb180436 |
---|---|
Category | Proteins |
Description | Recombinant Human 4-1BB ligand is produced by our E.coli expression system and the target gene encoding Arg71-Glu254 is expressed with a 6His tag at the C-terminus. |
Tag | C-His |
Form/Appearance | Lyophilized from a 0.2 um filtered solution of 20mM Tris HCl,150mM NaCl,pH7.4. |
UniProt ID | P41273 |
Application notes | 6His tag at the C-terminus. |
Expression System | E.coli |
Endotoxins | Less than 0.1 ng/ug (1 EU/ug) |
Biological Origin | Human |
Expression Region | Arg71-Glu254 |
Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Alternative names | Tumor necrosis factor ligand superfamily member 9; Read more... |
Note | For research use only |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 49.8 kDa after removal of the signal peptide.The apparent molecular mass of mFc-4-1BB Ligand-His is approximately 53-70 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result. |
Greater than 90% as determined by SDS-PAGE. | |
25.3 kDa | |
E.coli |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 49.1 kDa after removal of the signal peptide. The apparent molecular mass of hFc-m4-1BB Ligand is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |