Human TNF-beta / Lymphotoxin-alpha Protein, His Tag (MALS verified)

• Catalog Number: orb1733281
• Category: Proteins
• Description: The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 20.5 kDa
• Target: TNF-beta
• Source: Human TNF-beta Protein, His Tag (orb1733281) is expressed from human 293 cells (HEK293). It contains AA Leu 35 - Leu 205 (Accession # P01374).
• Expression System: HEK293
• Biological Activity: Immobilized Human TNFR1, Fc Tag at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, His Tag with a linear range of 1-20 ng/mL (QC tested).
• Expression Region: Leu 35 - Leu 205
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.5 kDa. The protein migrates as 26-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human TNFR1, Fc Tag (Cat. No. orb257878) at 5 μg/mL (100 μL/well) can bind Human TNF-beta Protein, His Tag (Cat. No. orb1733281) with a linear range of 1-20 ng/mL (QC tested).