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Catalog Number | orb49692 |
---|---|
Category | Proteins |
Description | Recombinant Human Triosephosphate Isomerase is produced by our E.coli expression system and the target gene encoding Met1-Gln249 is expressed with a 6His tag at the N-terminus. |
Tag | N-His |
Form/Appearance | Supplied as a 0.2 um filtered solution of 20mM Tris-HCl, 1mM DTT, 10% Glycerol, pH 8.0. |
UniProt ID | P60174 |
Expression System | E.coli |
Biological Origin | Human |
Expression Region | Met1-Gln249 |
Endotoxins | Less than 0.1 ng/ug (1 EU/ug) |
Storage | Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles. |
Alternative names | Triosephosphate Isomerase; TIM; Triose-Phosphate I Read more... |
Note | For research use only |
Application notes | 6His tag at the N-terminus. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 30.3 kDa after removal of the signal peptide. The apparent molecular mass of TIM1-His is approximately 100-130 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
ELISA, FC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
Greater than 85% as determined by SDS-PAGE. | |
59.1 kDa | |
Mammalian cell |
Greater than 85% as determined by SDS-PAGE. | |
37.4 kDa | |
E.coli |