Human Thrombopoietin / TPO Protein

• Catalog Number: orb867314
• Category: Proteins
• Description: Human Thrombopoietin Protein, Tag Free (THN-H5214) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Gly 353 (Accession # P40225-1).
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Conjugation: Unconjugated
• MW: 35.6 kDa
• Target: Thrombopoietin
• UniProt ID: P40225
• Source: Human Thrombopoietin, premium grade (orb867314) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Gly 353 (Accession # P40225-1).
• Expression System: HEK293
• Biological Activity: Human Thrombopoietin R, His Tag captured on CM5 Chip via anti-His antibody can bind Human Thrombopoietin, premium grade with an affinity constant of 14.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Ser 22 - Gly 353
• Endotoxins: 0.1 EU per μg
• NCBI: NP_000451.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: THPO,MGC163194,MGDF,MKCSF,ML,MPLLG,TPO,THCYT1
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 35.6 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Human Thrombopoietin R, His Tag (Cat. No. orb1101194) captured on CM5 Chip via anti-His antibody can bind Human Thrombopoietin, premium grade (Cat. No. orb867314) with an affinity constant of 14.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).