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Catalog Number | orb1173337 |
---|---|
Category | Assays and Kits |
Description | Human TGF-β1 ELISA Kit employs a two-site sandwich ELISA to quantitate Human TGF-β1. |
Tested applications | ELISA |
Reactivity | Human, Mouse, Rat |
Tag | TGFB1 |
Entrez | 7040 |
UniProt ID | P01137 |
Storage | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
Alternative names | TGFB1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 p Read more... |
Note | For research use only |
Application notes | Human TGF-β1 ELISA Kit employs a two-site sandwich ELISA to quantitate TGF-β1 in samples. An antibody specific for TGF-β1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGF-β1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGF-β1 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGF-β1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Sample Types | Cell culture supernatants#Serum#Plasma#Other biological fluids |
Expiration Date | Please enquire. |
Typical standard curve(R2>0.99)
Human | |
0.78 ng/mL-50 ng/mL | |
0.747 ng/mL |
Human | |
0.156 ng/mL-10 ng/mL | |
0.039 ng/mL |
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