Human TGF-Beta 1 / TGFB1 Protein

• Catalog Number: orb257996
• Category: Proteins
• Description: Transforming growth factor beta 1 ( TGFB1) is also known as TGF-β1, CED, DPD1, TGFB. is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The TGFB1 protein helps control the growth and division (proliferation) of cells, the process by which cells mature to carry out specific functions (differentiation), cell movement (motility), and the self-destruction of cells (apoptosis). The TGFB1 protein is found throughout the body and plays a role in development before birth, the formation of blood vessels, the regulation of muscle tissue and body fat development, wound healing, and immune system function. TGFB1 is particularly abundant in tissues that make up the skeleton, where it helps regulate bone growth, and in the intricate lattice that forms in the spaces between cells (the extracellular matrix). Within cells, this protein is turned off (inactive) until it receives a chemical signal to become active. TGFB1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGFB1. TGFB1 has been shown to interact with TGF beta receptor 1, LTBP1, YWHAE, EIF3I and Decorin.
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 12.8 kDa (monomer)
• Target: TGF-beta 1
• Source: Human TGFB1, premium grade (orb257996) is expressed from human 293 cells (HEK293). It contains AA Ala 279 - Ser 390 (Accession # P01137-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human TGFB1, premium grade at 0.2 μg/mL (100 μL/well) can bind Human TGFBR2, Fc Tag with a linear range of 0.2-2.5 ng/mL (QC tested).
• Expression Region: Ala 279 - Ser 390
• Endotoxins: 0.1 EU per μg
• NCBI: NP_000651.3
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 12 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: 0.085% TFA in 30% ACN
• Alternative names: CEDLAP,TGF-beta 1,TGFB1,DPD1,TGF-beta-1,TGFB
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 12.8 kDa (monomer). the protein migrates as 14 kDa (monomer) under reducing (R) condition, and 25 kDa (Dimer) under non-reducing (NR) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

References

Cirka, Heather et al. Active Traction Force Response to Long-Term Cyclic Stretch Is Dependent on Cell Pre-stress Biophys J, 110, 1845-1857 (2016)

PubMed: 27119644

SDS-PAGE analysis of Human TGF-beta 1 protein

Immobilized Human TGFB1, premium grade (Cat. No. orb257996) at 0.2 μg/mL (100 μL/well) can bind Human TGFBR2, Fc Tag (Cat. No. orb257866) with a linear range of 0.2-2.5 ng/mL (QC tested).