Human SOST / Sclerostin Protein, His Tag

• Catalog Number: orb257550
• Category: Proteins
• Description: Sclerostin (SOST) is also known as Sclerosteosis, VBCH, is a secreted glycoprotein with a signal peptide for secretion and a C-terminal cysteine knot-like (CTCK) domain and belongs to the Cerberus/DAN family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. More recently Sclerostin has been identified as binding to LRP5/6 receptors and inhibiting the Wnt signalling pathway. Wnt pathway inhibition under these circumstances is antagonistic to bone formation (meaning Sclerostin antagonizes bone formation). It has been shown that SOST binds BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. Sclerostin production by osteocytes is inhibited by parathyroid hormone, mechanical loading and cytokines including oncostatin M, cardiotrophin-1 and leukemia inhibitory factor. Sclerostin production is increased by calcitonin. Thus, osteoblast activity is self regulated by a negative feedback system. Mutations of Sclerostin is associated with the syndrome Sclerosteosis, and reduced sclerostin expression results in a milder form of the disorder called van Buchem disease.
• Reactivity: Human
• Tag: N-6×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 22.3 kDa
• Target: SOST
• Protein Sequence: AAI01087.1
• Source: Human SOST, His Tag (orb257550) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Tyr 213 (Accession # AAI01087.1).
• Expression System: HEK293
• Expression Region: Gln 24 - Tyr 213
• Endotoxins: 1.0 EU per μg
• NCBI: AAI01087.1
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: SOST,VBCH
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 22.3 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Wu, Y. et al. Upregulated serum sclerostin level in the T2DM patients with femur fracture inhibits the expression of bone formation/remodeling-associated biomarkers via antagonizing Wnt signaling Eur Rev Med Pharmacol Sci, 21, 470-478 (2017)

PubMed: 28239825

SDS-PAGE analysis of Human SOST protein