Human SOD1 / Cu-Zn SOD Protein

• Catalog Number: orb257851
• Category: Proteins
• Description: Superoxide dismutase [Cu-Zn] (SOD1) is also known as superoxide dismutase 1 (hSod1), an enzyme that in humans is encoded by the SOD1 gene, located on chromosome 21. SOD1 can bind copper and zinc ions and is one of three superoxide dismutases responsible for destroying free superoxide radicals in the body. The encoded isozyme (SOD1) is a soluble cytoplasmic and mitochondrial intermembrane space protein, acting as a homodimer to convert naturally occurring, but harmful, superoxide radicals to molecular oxygen and hydrogen peroxide. Furthermore, the mutations of SOD1 gene can result in a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor neurons in the brain stem and spinal cord.
• Reactivity: Human
• Tag: N-6×His
• Form/Appearance: Powder
• Purity: 97%
• Conjugation: Unconjugated
• MW: 16.8 kDa
• Target: SOD1
• Protein Sequence: AAH01034
• Source: Human SOD1, His Tag (orb257851) is expressed from E.coli cells. It contains AA Ala 2 - Gln 154 (Accession # P60052-1).
• Expression System: E. coli
• Expression Region: Ala 2 - Gln 154
• Endotoxins: 1.0 EU per μg
• NCBI: AAH01034
• Storage: -20℃
• Buffer/Preservatives: 50 mM Tris, 150 mM NaCl, pH7.5
• Alternative names: SOD1,Superoxide dismutase 1,hSod1
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 19-21 kDa under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

References

Sakanyan, Vehary et al. Activation of EGFR by small compounds through coupling the generation of hydrogen peroxide to stable dimerization of Cu/Zn SOD1 Sci Rep, 6, 21088 (2016)

PubMed: 26883293

SDS-PAGE analysis of Human SOD1 protein