Human SMAC / Diablo Protein

• Catalog Number: orb257394
• Category: Proteins
• Description: Diablo homolog, mitochondrial (DIABLO) is also known as direct IAP-binding protein with low pI and second mitochondria-derived activator of caspase (SMAC), which can interact with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Acting by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP),DIABLO can also promote apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Also, DIABLO inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Furthermore, DIABLO is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 21.7 kDa
• Target: SMAC
• Protein Sequence: NP_063940
• Source: Human SMAC, His Tag (orb257394) is expressed from E.coli cells. It contains AA Ala 56 - Asp 239 (Accession # NP_063940).
• Expression System: E. coli
• Expression Region: Ala 56 - Asp 239
• Endotoxins: 1.0 EU per μg
• NCBI: NP_063940
• Storage: -20℃
• Buffer/Preservatives: 20 mM HEPES, 150 mM NaCl, pH7.5
• Alternative names: DIABLO,SMAC
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 21.7 kDa. The protein migrates as 22 kDa under reducing (R) condition (SDS-PAGE).
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human SMAC protein