Human Semaphorin 4D / SEMA4D / CD100 Protein

• Catalog Number: orb257252
• Category: Proteins
• Description: Cluster of Differentiation 100 (CD100) is also known as Semaphorin-4D (SEMA4D), is a single-pass type I membrane protein which belongs to the semaphorin family, and is a human 150-kDa homodimer expressed at the surface of most hemopoietic cells. Semaphorin genes encode soluble and membrane-bound proteins, most of which have been shown to act as chemorepellents on growth cone guidance. CD100 is discrete, as it is a transmembrane leukocyte surface molecule that can also exist in a soluble form. Semaphorin 4D (Sema 4D) is an axon guidance molecule which is secreted by oligodendrocytes and induces growth cone collapse in the central nervous system. By binding plexin B1 receptor it functions as an R-Ras GTPase-activating protein (GAP) and repels axon growth cones in both the mature central nervous system. In the immune system, CD100 binds CD72 to activate B cells and dendritic cells, though much about this interaction is still under investigation. It is involved in oligodendrogenesis during development and during recovery from ischemic injury.
• Reactivity: Human
• Tag: C-Fc
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 105.4 kDa
• Target: Semaphorin 4D
• Protein Sequence: AAH54500
• Source: Human SEMA4D, Fc Tag (orb257252) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Arg 734 (Accession # AAH54500).
• Expression System: HEK293
• Expression Region: Met 22 - Arg 734
• Endotoxins: 1.0 EU per μg
• NCBI: AAH54500
• Storage: -20℃
• Buffer/Preservatives: 50 mM Tris, 100 mM Glycine, pH7.5
• Alternative names: SEMA4D,C9orf164,CD100,FLJ33485,FLJ34282,FLJ39737,F
• Note: For research use only
• Application notes: This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 105.4 kDa. The protein migrates as 125-160 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Zou, Ting et al. Semaphorin 4D Enhances Angiogenic Potential and Suppresses Osteo-/Odontogenic Differentiation of Human Dental Pulp Stem Cells J Endod, 43, 297-305 (2017)

PubMed: 28027822

SDS-PAGE analysis of Human Semaphorin 4D protein