Human Semaphorin 4A / SEMA4A Protein

• Catalog Number: orb257835
• Category: Proteins
• Description: Semaphorin-4A (SEMA4A) is also known as Semaphorin-B (SEMAB), which belongs to the semaphorin family. SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one sema domain. SEMA4A is cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. SEMA4A plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. SEMA4A also promotes phosphorylation of TIMD2 and inhibits angiogenesis. SEMA4A promotes axon growth cone collapse and inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 72.7 kDa
• Target: Semaphorin 4A
• Protein Sequence: AAH20974
• Source: Human Semaphorin 4A, His Tag (orb257835) is expressed from human 293 cells (HEK293). It contains AA Gly 33 - His 683 (Accession # AAH20974).
• Expression System: HEK293
• Expression Region: Gly 33 - His 683
• Endotoxins: 1.0 EU per μg
• NCBI: AAH20974
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: SEMA4A,SEMAB,SEMB,Semaphorin-4A,Semaphorin-B
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 72.7 kDa. The protein migrates as 70-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Peng, Hai-Ying et al. Semaphorin 4A enhances lung fibrosis through activation of Akt via PlexinD1 receptor J Biosci, 40, 855-862 (2015)

PubMed: 26648031

SDS-PAGE analysis of Human Semaphorin 4A protein