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Catalog Number | orb867010 |
---|---|
Category | Assays and Kits |
Description | Sandwich Human SARS-CoV-2 Nucleocapsid ELISA Kit |
Tested applications | ELISA |
Reactivity | Human |
Range | 7.82-500 pg/mL |
Concentration | 500 pg/mL |
Sensitivity | 3.2 pg/mL |
Protein Length | 3.5h |
Alternative names | COVID-19;SARS-CoV-2; 2019-nCoV ELISA Kit Read more... |
Note | For research use only |
Application notes | standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SARS-CoV-2 Nucleocapsid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SARS-CoV-2 Nucleocapsid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SARS-CoV-2 Nucleocapsid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SARS-CoV-2 Nucleocapsid in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
Human | |
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