Human S100A2 ELISA Kit

• Catalog Number: orb777852
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A2(S100A2).
• Tested applications: ELISA
• Reactivity: Human
• Range: 0.32-20 ng/mL
• Concentration: 20 ng/mL
• UniProt ID: P29034
• Sensitivity: 0.113 ng/mL
• Protein Length: 3.5h
• Alternative names: S100-A2 ELISA Kit, CAN19 ELISA Kit, S100L ELISA Ki
• Note: For research use only
• Application notes: Standard: 20 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S100A2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S100A2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S100A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S100A2 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.