Human ROR1

SKU: orb2994090

Description

Recombinant Human Inactive Tyrosine-protein Kinase Transmembrane Receptor ROR1 is produced by our Mammalian expression system and the target gene encoding Gln30-Glu403 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human ROR1 (C-6His)(Cat#orb2994090) at 2 μg/ml (100 μl/well) can bind Human ROR1 Antibody (3E8)(Cat#orb2992600). The ED50 of Human ROR1 Antibody (3E8)(Cat#orb2992600) is 1.69 ng/ml. (Regularly tested). BLI: Loaded Human ROR1 Antibody (3E8)-Fc(Cat#orb2992600) on Protein A Biosensor, can bind Human ROR1-His(Cat#orb2994090) with an affinity constant of 6.35 nM as determined in BLI assay. (Regularly tested) Loaded Recombinant Human ROR1 (C-6His) (Cat#orb2994090) on HIS1K Biosensor, can bind Human ROR1 Antibody(3E8) (Cat#orb2992600) with an affinity constant of 0.163 pM as determined in BLI assay. (Regularly tested)
• Tag: C-6His
• Molecular Weight: Predicted: 42.8 KDa. Observed: 60-80 KDa, reducing conditions
• Expression Region: Gln30-Glu403
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM Citrate, 5% Trehalose, 1mM EDTA, 0.02% Tween80, pH 5.5.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Inactive tyrosine-protein kinase transmembrane receptor ROR1; Neurotrophic tyrosine kinase, receptor-related 1; ROR1; NTRKR1

Quick Database Links

UniProt

Q01973