Human RENIN Protein

• Catalog Number: orb257792
• Category: Proteins
• Description: Renin is also known as REN and angiotensinogenase, is a circulating enzyme that participates in the body's renin-angiotensin system (RAS), and plays an essential role in the elevation of arterial blood pressure and increased sodium retention by the kidney. Renin activates the renin-angiotensin system by cleaving angiotensinogen, produced by the liver, to yield angiotensin I, which is further converted into angiotensin II by ACE, the angiotensin-converting enzyme primarily within the capillaries of the lungs. Renin is secreted from kidney cells, which are activated via signaling from the macula densa, which responds to the rate of fluid flow through the distal tubule, by decreases in renal perfusion pressure (through stretch receptors in the vascular wall), and by sympathetic nervous stimulation, mainly through beta-1 receptor activation. Renin can bind to ATP6AP2, which results in a fourfold increase in the conversion of angiotensinogen to angiotensin I over that shown by soluble renin. In addition, renin binding results in phosphorylation of serine and tyrosine residues of ATP6AP2. The level of renin mRNA appears to be modulated by the binding of HADHB, HuR and CP1 to a regulatory region in the 3' UTR. An over-active renin-angiotension system leads to vasoconstriction and retention of sodium and water. These effects lead to hypertension. Therefore, renin inhibitors can be used for the treatment of hypertension.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 43.1 kDa
• Target: RENIN
• Protein Sequence: AAH33474
• Source: Human RENIN, His Tag (orb257792) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Arg 406 (Accession # P00797-1).
• Expression System: HEK293
• Expression Region: Leu 24 - Arg 406
• Endotoxins: 1.0 EU per μg
• NCBI: AAH33474
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: REN,FLJ10761,Renin,angiotensinogenase
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 43.1 kDa. The protein migrates as 46-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human RENIN protein