Human R-Spondin 1 / RSPO1 Protein

• Catalog Number: orb257807
• Category: Proteins
• Description: R-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), which is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns. Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furinlike domains are needed for β-catenin stabilization. A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 28.7 kDa
• Target: R-Spondin 1
• Protein Sequence: NP_001033722.1
• Source: Human R-Spondin 1, His Tag (orb257807) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ala 263 (Accession # Q2MKA7-1).
• Expression System: HEK293
• Expression Region: Ser 21 - Ala 263
• Endotoxins: 1.0 EU per μg
• NCBI: NP_001033722.1
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: RSPO1,CRISTIN3,FLJ40906,RP11-566C13.1,R-spondin-1
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 28.7 kDa. The protein migrates as 38-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Das, Indrajit et al. Glucocorticoids alleviate intestinal ER stress by enhancing protein folding and degradation of misfolded proteins J Exp Med, 210, 1201-1216 (2013)

PubMed: 23650437

SDS-PAGE analysis of Human R-Spondin 1 protein