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Human Protease Activated Receptor 2 (PAR2) ELISA Kit

Catalog Number: orb1949142

DispatchUsually dispatched within 5-10 working days
$ 280.00
Catalog Numberorb1949142
CategoryAssays and Kits
DescriptionHuman Protease Activated Receptor 2 (PAR2) ELISA Kit
ReactivityHuman
Range0.16-10ng/mL
Assay Time3.5H
TargetPAR2
UniProt IDP55085
Sensitivity0.1 ng/mL
Alternative namesF2RL1, F2-RL1, GPR11, PAR2, Coagulation Factor II(
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NoteFor research use only
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PAR2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PAR2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PAR2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PAR2. You can calculate the concentration of Human PAR2 in the samples by comparing the OD of the samples to the standard curve.
Sample TypesTissue homogenate, Cell lysate
Expiration DatePlease enquire.
Human Protease Activated Receptor 2 (PAR2) ELISA Kit