Human Prolactin / PRL Protein

• Catalog Number: orb511283
• Category: Proteins
• Description: Prolactin (gene name PRL) is a secreted neuroendocrine pituitary hormone that acts primarily on the mammary gland to promote lactation, but has pleiotropic effects in both males and females. Prolactin is synthesized as a prohormone. Following cleavage of the signal peptide, the length of the mature hormone is between 194 and 199 amino acids, depending on species. Hormone structure is stabilized by three intramolecular disulfide bonds. Excessive secretion of prolactin - hyperprolactinemia - is a relative common disorder in humans. This condition has numerous causes, including prolactin-secreting tumors and therapy with certain drugs. The prolactin receptor (gene name PRLR) is a transmembrane type I glycoprotein that belongs to the cytokine hematopoietic receptor family. Expression of the prolactin receptor is widespread. Each prolactin molecule is thought to bind two receptor molecules
• Reactivity: Human
• Tag: C-mFc(mIgG2a)
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 49.8 kDa
• Target: Prolactin
• Source: Human Prolactin Protein, Mouse IgG2a Fc Tag (orb511283) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Cys 227 (Accession # Q5THQ0-1).
• Expression System: HEK293
• Biological Activity: Immobilized Human Prolactin, Mouse IgG2a Fc Tag, low endotoxinat 1 μg/mL (100 μL/well) can bind Human Prolactin R / PRLR Protein, Fc Tag with a linear range of 2-78 ng/mL (QC tested).
• Expression Region: Leu 29 - Cys 227
• Endotoxins: 0.1 EU per μg
• NCBI: NP_000939.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: Tris with Glycine, Arginine and NaCl, pH7.5
• Alternative names: PRL,Prolactin
• Note: For research use only
• Application notes: This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 49.8 kDa. The protein migrates as 50-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Human Prolactin, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. orb511283) at 1 μg/mL (100 μL/well) can bind Human Prolactin R / PRLR Protein, Fc Tag (Cat. No. orb420091) with a linear range of 2-78 ng/mL (QC tested).