Biotinylated Human PD-L1

SKU: orb2994143

Description

Biotinylated Recombinant Human Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a human IgG1 Fc, Avi tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Recombinant Human PD-1-His(Cat#orb2993958) at 5μg/ml (100 μl/well)can bind Human PD-L1-Fc-Avi(Cat#orb2994143). The ED50 of Human PD-L1-Fc-Avi(Cat#orb2994143) is 0.185 ug/ml.. BLI: Loaded Mouse PD-1-His(Cat#orb2994450) on HIS1K Biosensor, can bind Biotinylated Human PD-L1-Fc-Avi (Cat#orb2994143) with an affinity constant of -3 nM as determined in BLI assay.
• Tag: C-Fc-Avi
• Molecular Weight: Predicted: 54.2 KDa. Observed: 70-95 KDa, reducing conditions
• Expression Region: Phe19-Thr239
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Biotin

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1

Quick Database Links

UniProt

Q9NZQ7