Human PLAU / uPA Protein

• Catalog Number: orb257779
• Category: Proteins
• Description: Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 92%
• Conjugation: Unconjugated
• MW: 47.2 kDa
• Target: PLAU
• Protein Sequence: NP_002649.1
• Source: Human PLAU, His Tag (orb257779) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431 (Accession # NP_002649.1).
• Expression System: HEK293
• Expression Region: Ser 21 - Leu 431
• Endotoxins: 1.0 EU per μg
• NCBI: NP_002649.1
• Storage: -20℃
• Buffer/Preservatives: 50 mM Sodium Acetate, 100 mM NaCl, pH5.0
• Alternative names: Urokinase,PLAU,ATF,UPA,URK,u-PA,BDPLT5,QPD
• Note: For research use only
• Application notes: Human PLAU, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 47.2 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. Please note that the protein is not activated by protease digestion. In vitro activation is generally recommended for higher activity.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human PLAU protein