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Catalog Number | orb1088173 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prostaglandin H2(PGH2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostaglandin H2(PGH2). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin H2(PGH2) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Human |
Range | 3.13-200 pg/mL |
Sensitivity | 0.97 pg/mL |
Protein Length | 2h |
Alternative names | PG-H2 Read more... |
Note | For research use only |
Sample Types | Serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
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