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Catalog Number | orb1088176 |
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Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prostaglandin F2 Alpha(PGF2a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prostaglandin F2 Alpha(PGF2a). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin F2 Alpha(PGF2a) in the samples is then determined by comparing the OD of the samples to the standard curve. |
Reactivity | Human |
Range | 12.5-800 pg/mL |
Sensitivity | 3.88 pg/mL |
Protein Length | 2h |
Alternative names | PGF2A, PGF2-A, PG-F2a Read more... |
Note | For research use only |
Sample Types | serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
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