Human PD-L1

SKU: orb2994368

Description

Recombinant Human Programmed Cell Death 1 Ligand 1 is produced by our Mammalian expression system and the target gene encoding Phe19-Thr239 is expressed with a Flag tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human PD-L1-Flag(Cat#orb2994368) at 2μg/ml (100 μl/well)can bind Anti-Human PDL1 mAb-Fc(Cat#orb2993144).The ED50 of Anti-Human PDL1 mAb-Fc(Cat#orb2993144) is 14.68 ng/ml.. BLI: Loaded Human PD-1-Fc(Cat#orb2994333) on Protein A Biosensor, can bind Human PD-L1-Flag(Cat#orb2994368) with an affinity constant of 0.19 uM as determined in BLI assay.
• Tag: C-Flag
• Molecular Weight: Predicted: 26.3 KDa. Observed: 35-40 KDa, reducing conditions
• Expression Region: Phe19-Thr239
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Disclaimer: For research use only

Alternative Names

Programmed Cell Death 1 Ligand 1; PD-L1; PDCD1 Ligand 1; Programmed Death Ligand 1; B7 Homolog 1; B7-H1; CD274; B7H1; PDCD1L1; PDCD1LG1; PDL1

Quick Database Links

UniProt

Q9NZQ7