Human Podocalyxin (PCX) ELISA Kit

SKU: orb775909

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Podocalyxin(PCX).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PCX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PCX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PCX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PCX in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

Gp200 ELISA Kit, PCLP ELISA Kit, PC ELISA Kit, PODXL ELISA Kit, Podocalyxin Like Protein 1 ELISA Kit, GCTM-2 antigen ELISA Kit

Quick Database Links

UniProt

O00592

References

C Li, JKC Ng, GCK Chan, WWS Fung, KM Chow, CC Szeto Preservation of Urinary Podocyte Markers in Diabetic Kidney Disease by SGLT2 Inhibitor Therapy Kidney Diseases, (2025)

PubMed:

Applications

ELISA

Reactivity

Human