Human PCSK9 (D374Y) Protein

• Catalog Number: orb257745
• Category: Proteins
• Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 90%
• Conjugation: Unconjugated
• MW: 13.8 kDa and 58.2 kDa
• Target: PCSK9
• Protein Sequence: AAI66619.1 (D374Y)
• Source: Human PCSK9 (D374Y), His Tag (orb257745) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1 (D374Y)).
• Expression System: HEK293
• Expression Region: Gln 31 - Gln 692
• Endotoxins: 1.0 EU per μg
• NCBI: D374, AAI66619.1
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 58.2 kDa respectively. The protein migrates as 17 kDa and 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The D374Y mutation results in higher affinity of PCSK9 for LDLR.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human PCSK9 protein

Line graph illustrates about the sensitivity range of Human PCSK9 protein using different concentrations