Human Osteoactivin / GPNMB Protein

• Catalog Number: orb257522
• Category: Proteins
• Description: Transmembrane glycoprotein NMB (GPNMB) is also known as Transmembrane glycoprotein HGFIN, DC-HIL and Osteoactivin (OA), which belongs to the PMEL/NMB family. GPNMB contains one PKD domain. GPNMB is a transmembrane glycoprotein that is up-regulated in various cancer cells, including in glioblastoma multiforme and is expressed in many melanoma cells, as well as in tissue macrophages. GPNMB protein acts as a downstream mediator of BMP-2 effects on osteoblast differentiation and function. GPNMB participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 53.4 kDa
• Target: Osteoactivin
• Source: Human Osteoactivin, His Tag (orb257522) is expressed from human 293 cells (HEK293). It contains AA Ala 22 - Pro 486 (Accession # AAH32783).
• Expression System: HEK293
• Expression Region: Ala 22 - Pro 486
• Endotoxins: 1.0 EU per μg
• NCBI: AAH32783
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: GPNMB,HGFIN,NMB,Osteoactivin
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.4 kDa. The protein migrates as 85-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Arosarena, Oneida A. et al. Osteoactivin regulates head and neck squamous cell carcinoma invasion by modulating matrix metalloproteases J Cell Physiol, 233, 409-421 (2018)

PubMed: 28295306

SDS-PAGE analysis of Human Osteoactivin protein