Human Neprilysin / MME / CD10 Protein

• Catalog Number: orb257693
• Category: Proteins
• Description: Cluster of differentiation 10 (CD10) is also known as membrane metallo-endopeptidase, neutral endopeptidase (NEP), Neprilysin, and common acute lymphoblastic leukemia antigen (CALLA), is a 90-110-kDa type II transmembrane glycoprotein normally expressed by a variety of tissues, including epithelial cells of the prostate, kidney, intestine, endometrium, adrenal glands, and lung. This zinc-dependent metalloprotease enzyme cleaves peptide bonds on the amino side of hydrophobic residues and inactivates a variety of physiologically active secreted peptides. CD20 is thought to be the rate-limiting degrading enzyme of amyloid β peptide (Aβ) whose abnormal misfolding and aggregation in neural tissue has been implicated in the development of Alzheimer's disease (AD). CD10 is also identified as the common acute lymphoblastic leukemia antigen (CALLA) present on leukemic cells of pre-B phenotype, and thus serves as the most important biomarker in the diagnosis of human acute lymphocytic leukemia (ALL).
• Reactivity: Human
• Tag: Native
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 80.0 kDa
• Target: Neprilysin
• Protein Sequence: NP_000893.2
• Source: Human Neprilysin, Tag Free (orb257693) is expressed from human 293 cells (HEK293). It contains AA Tyr 52 - Trp 750 (Accession # NP_000893.2).
• Expression System: HEK293
• Expression Region: Tyr 52 - Trp 750
• Endotoxins: 1.0 EU per μg
• NCBI: NP_000893.2
• Storage: -20°C
• Buffer/Preservatives: 20 mM Tris, 100 mM NaCl, pH8.0
• Alternative names: MME,CALLA,CD10,DKFZp686O16152,MGC126681,MGC126707,
• Note: For research use only
• Application notes: This protein carries no "tag". The protein has a calculated MW of 80.0 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

References

Willard, Joshua R. et al. Improved glycaemia in high-fat-fed neprilysin-deficient mice is associated with reduced DPP-4 activity and increased active GLP-1 levels Diabetologia, 60, 701-708 (2017)

PubMed: 27933334

SDS-PAGE analysis of Human Neprilysin protein