Human MMP-1 Protein, His Tag (active enzyme, MALS verified) [Out of stock]

• Catalog Number: orb1743007
• Category: Proteins
• Description: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of matrix metalloproteinases (MMPs) 2 and 9, which are key effectors of ECM remodeling. Matrix metalloproteinase-1 (MMP-1) is also known as interstitial collagenase and fibroblast collagenase. MMP­1 is expressed by fibroblasts, keratinocytes, endothelial cells, monocytes and macrophages. MMP-1 breaks down the interstitial collagens, types I, II, and III. MMP­1 can degrade a broad range of substrates including types I, II, III, VII, VIII, and X collagens as well as casein, gelatin, myelin basic protein, L­Selectin, pro­TNF, IL­1β, IGF­BP3, IGF­BP5, pro MMP­2 and pro MMP­9.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Liquid
• Purity: 95%
• Conjugation: Unconjugated
• MW: 53.7 kDa
• Target: MMP-1
• Source: Human MMP-1 Protein, His Tag (orb1743007) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Asn 469 (Accession # P03956-1).
• Expression System: HEK293
• Expression Region: Phe 20 - Asn 469
• Endotoxins: 1.0 EU per μg
• Storage: -70°C
• Buffer/Preservatives: 25 mM MES, 150 mM NaCl, pH 6.0
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 53.7 kDa. The protein migrates as 51-61 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.