Human MICA Protein

Catalog Number: orb257689

Catalog Number	orb257689
Category	Proteins
Description	MHC class I polypeptide-related sequence A (MICA) belongs to the MHC class I family and MIC subfamily. MICA contains one Ig-like C1-type (immunoglobulin-like) domain. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. MICA is ligand for the KLRK1/NKG2D receptor. MICA bind to KLRK1 leads to cell lysis.
Reactivity	Human
Tag	C-7×His
Form/Appearance	Powder
Purity	95%
Conjugation	Unconjugated
MW	33.8 kDa
Target	MICA
Protein Sequence	AAH16929.1
Source	Human MICA, His Tag (orb257689) is expressed from human 293 cells (HEK293). It contains AA Glu 24 - Gln 308 (Accession # AAH16929.1).
Expression System	HEK293
Expression Region	Glu 24 - Gln 308
Endotoxins	1.0 EU per μg
NCBI	AAH16929.1
Storage	-20℃
Buffer/Preservatives	PBS, pH7.4
Alternative names	MIC-A Read more...
Note	For research use only
Application notes	This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.8 kDa. The protein migrates as 45-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Expiration Date	6 months from date of receipt.

SDS-PAGE analysis of Human MICA protein

Conjugation:	Unconjugated
Purity:	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
MW:	The protein has a predicted molecular mass of 33.5 kDa after removal of the signal peptide. The apparent molecular mass of MICA-His is approximately 55-70 kDa due to glycosylation.
Source:	Mammalian

Sizes Available: 100 μg, 10 μg, 50 μg

Applications:	ELISA, MS, SDS-PAGE, WB
Purity:	Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Source:	Human cells