Human Membrane-Spanning 4-Domains, Subfamily A, Member 4 (MS4A4A) ELISA Kit

SKU: orb1173605

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to membrane-spanning 4-domains, subfamily A, member 4(MS4A4A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to membrane-spanning 4-domains, subfamily A, member 4(MS4A4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain membrane-spanning 4-domains, subfamily A, member 4(MS4A4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of membrane-spanning 4-domains, subfamily A, member 4(MS4A4A) in the samples is then determined by comparing the OD of the samples to the standard curve.

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MS4A4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MS4A4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MS4A4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MS4A4A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.3 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

4SPAN1, CD20 antigen like 1, CD20L1, MS4A4,

Quick Database Links

UniProt

Q96JQ5