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Catalog Number | orb223929 |
---|---|
Category | Proteins |
Description | Recombinant Human ME2 His tag protein |
Tested applications | SDS-PAGE |
Reactivity | Human |
Purity | > 95% by SDS-PAGE and HPLC analyses. |
Conjugation | Unconjugated |
MW | 64.4 kDa |
Target | Human ME2 His tag |
Source | E. coli |
Biological Activity | Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 iO 0.6 mM. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | Sterile Filtered White lyophilized (freeze-dried) powder. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
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