Human MANF Protein, His Tag (MALS verified)

• Catalog Number: orb1570930
• Category: Proteins
• Description: MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote the survival, growth and function of dopamine-specific neurons. MANF and its structural homolog CDNF each contain a N-terminal, saposin-like, lipid-binding domain, and a carboxyl-terminal domain that is not homologous to previously characterized protein structures. MANF and CDNF can prevent 6-OHDA-induced degeneration of dopaminergic neurons by triggering survival pathways in a rat experimental model of Parkinson's disease. Recombinant Human MANF is an 18.1 kDa protein consisting of 158 amino acids, including 8 cysteine residues.
• Reactivity: Human
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 20.0 kDa
• Target: MANF
• Protein Sequence: Leu 25 - Leu 182
• Source: Human MANF Protein, His Tag (orb1570930) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Leu 182 (Accession # P55145).
• Expression System: HEK293
• Expression Region: Leu 25 - Leu 182
• Endotoxins: 0.1 EU per μg
• Storage: -20℃
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: Mesencephalic astrocyte-derived neurotrophic facto
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.0 kDa. The protein migrates as 19-21 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.