Human M-CSF R / CSF1R / CD115 Protein

• Catalog Number: orb257371
• Category: Proteins
• Description: Colony stimulating factor 1 receptor (CSF1R) is also known as macrophage colony-stimulating factor receptor (M-CSFR), CD115 Cluster of Differentiation 115 (CD115), C-FMS, CSFR, FIM2, FMS, and is a member of the typeⅢ subfamily of receptor tyrosine kinases (RTKs). CSF1R is a receptor for a cytokine called colony stimulating factor 1, The protein encoded by the CSFR1 gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most, if not all, of the biological effects of this cytokine. Ligand binding activates CSFR1 through a process of oligomerization and transphosphorylation . Mutations in CSF1R are associated with chronic myelomonocytic leukemia and type M4 acute myeloblastic leukemia. Increased levels of CSF1R1 are found in microglia in Alzheimer's disease and after brain injuries. The increased receptor expression causes microglia to become more active. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.
• Reactivity: Human
• Tag: C-6×His
• Form/Appearance: Powder
• Purity: 96%
• Conjugation: Unconjugated
• MW: 55.3 kDa
• Target: M-CSF R
• Source: Human M-CSF R, His Tag (orb257371) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # NP_005202.2).
• Expression System: HEK293
• Biological Activity: Biotinylated Human M-CSF, His, immobilized on SA Chip can bind Human M-CSF R, His Tag with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Ile 20 - Glu 512
• Endotoxins: 1.0 EU per μg
• NCBI: NP_005202.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 55.3 kDa. The protein migrates as 80-95 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Human M-CSF R protein

Biotinylated Human M-CSF, His, Avitag immobilized on SA Chip can bind Human M-CSF R, His Tag (Cat. No. orb257371) with an affinity constant of 0.309 μM as determined in a SPR assay (Biacore T200) (Routinely tested).