Human M-CSF / CSF-1 Protein

• Catalog Number: orb402822
• Category: Proteins
• Description: The colony stimulating factor 1 (CSF1), also known as macrophage colony-stimulating factor (M-CSF), is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types. Eukaryotic cells also produce M-CSF in order to combat intercellular viral infection. It is one of the three experimentally described colony-stimulating factors. M-CSF binds to the colony stimulating factor 1 receptor. Macrophage colony-stimulating factor has been shown to interact with PIK3R2. M-CSF (or CSF-1) is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. Locally produced M-CSF in the vessel wall contributes to the development and progression of atherosclerosis.
• Reactivity: Human
• Tag: N-6×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 26.0 kDa
• Target: M-CSF
• Source: Human M-CSF, His Tag (orb402822) is expressed from human 293 cells (HEK293). It contains AA Glu 33 - Arg 255 (Accession # P09603-1).
• Expression System: HEK293
• Biological Activity: Human M-CSF, His Tag captured on CM5 Chip via anti-His antibody can bind Human M-CSF R, Fc Tag, low endotoxinwith an affinity constant of 1.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).
• Expression Region: Glu 33 - Arg 255
• Endotoxins: 1.0 EU per μg
• NCBI: AAH21117.1
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: M-CSF,CSF-1,Lanimostim
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 26.0 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Human M-CSF, His Tag (Cat. No. orb402822) captured on CM5 Chip via anti-His antibody can bind Human M-CSF R, Fc Tag, low endotoxin (Cat. No. orb257372) with an affinity constant of 1.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).