Human Laminin Alpha 4 (LAMa4) ELISA Kit

SKU: orb777902

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 4(LAMa4).

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAMa4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAMa4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAMa4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAMa4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.63-40 ng/mL
• Sensitivity: 0.243 ng/mL
• Concentration: 40 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

LAM-A4 ELISA Kit, LAMA3 ELISA Kit

Quick Database Links

UniProt

Q16363