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Human Laminin Alpha 3 (LAMa3) ELISA Kit

Catalog Number: orb777719

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb777719
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Alpha 3(LAMa3).
ReactivityHuman
Concentration1000 pg/mL
UniProt IDQ16787
Tested applicationsELISA
Application notesstandard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAMa3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAMa3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAMa3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAMa3 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range15.63-1000 pg/mL
Sensitivity6 pg/mL
Sample TypesSerum, plasma, tissue homogenates and other biological fluids.
Alternative namesLAM-A3 ELISA Kit, E170 ELISA Kit, LAMNA ELISA Kit,
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NoteFor research use only
Human Laminin Alpha 3 (LAMa3) ELISA Kit

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