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Human Lambda chain Mouse Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution range | IHC-P=1:200-1000, WB=1:500-2000, IHC-F=1:200-1000, IF=1:200-1000 |
| Reactivity | Human |
| Predicted Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 7D1 |
| Immunogen | Purified native protein |
| Target | IGLC1 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M PBS (pH7.4). |
| Disclaimer | For research use only |
Alternative Names
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Paraformaldehyde-fixed, paraffin embedded (human lymphoid), Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (orb1974610) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in E sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Human Lambda chain) Monoclonal Antibody, Unconjugated (orb1974610) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining.

Sample: Lane 1: Human Serum, Primary: Anti-Human Lambda chain (orb1974610) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: kDa, Observed band size: 25 kDa.
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Human Lambda chain Mouse Monoclonal Antibody (orb1974610)
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