Human L-Type Amino Acid Transporter 1 (y+LAT1) ELISA Kit

• Catalog Number: orb778573
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to L-Type Amino Acid Transporter 1(y+LAT1).
• Reactivity: Human
• Concentration: 20 ng/mL
• UniProt ID: Q9UM01
• Tested applications: ELISA
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human y+LAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human y+LAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human y+LAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human y+LAT1 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.118 ng/mL
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Alternative names: SLC7A7 ELISA Kit, LPI ELISA Kit, Y+LAT1 ELISA Kit,
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: Please enquire.