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Catalog Number | orb1088136 |
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Category | Tools |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KL-6 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Range | 12.5-800 IU/mL |
Sensitivity | 5.9 IU/mL |
Protein Length | 2h |
Alternative names | KL-6 Read more... |
Note | For research use only |
Sample Types | serum, plasma, tissue homogenates and other biological fluids |
Expiration Date | 12 months from date of receipt. |
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