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Human JNK(c-Jun N-terminal kinases) ELISA Kit

Catalog Number: orb1736655

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1736655
CategoryAssays and Kits
DescriptionThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human JNK in the samples is then determined by comparing the OD of the samples to the standard curve.
ReactivityHuman
Range0.32-20 ng/mL
Concentration20 ng/mL
UniProt IDP45983
Sensitivity0.14 ng/mL
Alternative namesMAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SA
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NoteFor research use only
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human JNK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human JNK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human JNK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human JNK in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typestissue homogenates, cell lysates and other biological fluids
Expiration DatePlease enquire.
Human JNK(c-Jun N-terminal kinases) ELISA Kit