Human Interferon-alpha 2b, Saccharomyces protein

• Catalog Number: orb80040
• Category: Proteins
• Description: Interferon-alpha 2b Human Recombinant produced in yeast is a single, glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of approximately 19 kDa.The Interferon-alpha 2b gene was obtained from human leukocytes.The IFN-a 2b is purified by proprietary chromatographic techniques.
• Target: IFN 2b, Yeast
• Conjugation: Unconjugated
• Buffer/Preservatives: Lyophilized from 0.2 um filtered concentrated (1mg/ml) solution in PBS, pH-7.4.
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Protein Sequence: CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRHDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE
• Tested applications: FA, HPLC, SDS-PAGE
• Application notes: < strong>Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IFN-a 2b as Reference Standard.
• Biological Origin: Saccharomyces cerevisiae
• Biological Activity: Fully biologically active when compared to standard. Fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay was found to be no less than 1.6 x 10⁸ IU/ mg.
• Solubility (25°C): It is recommended to reconstitute the lyophilized glycosilated IFN alpha 2b in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Alternative names: Interferon alpha 2b protein, IFNA protein, INFA2 p
• Note: For research use only